01/17/07 Introduction, Overview of Course & Visit to the NMR Facility (mg)

01/19/07 Basic Principles of NMR (sc)
(Lecture_Notes)
(Problem_Set_1)

01/22/07 Signal Properties and Data Processing (sc)
(Lecture_Notes)
(Problem_Set_2)

01/24/07 Chemical Shift and Spin-Spin Coupling and Introduction to T1 and T2 (mg)
(Lecture_Notes_and_Problem_Set_3)

01/26/07 Lab 1:Introduction & basic 1D experiments (sc) NOTE: in Ullmann B8 (NMR Facility)
(Lab_Notes)
(AECOM_DRX300_Reference_Guide)
(Lab_1_Problem_Set)

01/29/07 Relaxation & the NOE (mg)
(Lecture_Notes_and_Problem_Set_4)

01/31/07 Product operator description & coherence transfer (sc)
(Lecture_Notes)
(Product_Operators_Sheet)
(Problem_Set_5)

02/02/07 A repertoire of one-dimensional methods (mg)
(Lecture_Notes)

02/05/07 Pulsed field gradients, Solvent suppression and Self diffusion (mg)
(Lecture_Notes)
(Problem_Set_6)

02/07/07 Lab 2:1D Homonuclear and Heteronuclear Experiments (sc) NOTE: in Ullmann B8 (NMR Facility)
(Lab_Notes)
(Lab_2_Problem_Set)

02/09/07 Exchange & Measuring Dynamic Processes (sc)
(Lecture_Notes)

02/12/07 Introduction to two-dimensional NMR (mg)
(Lecture_Notes)
(Problem_Set_7)

02/14/07 2D Heteronuclear experiments (mg)
(Lecture_Notes) No problem set!

02/16/07 2D Homonuclear experiments (mg)
(Lecture_Notes) No problem set!

02/21/07 Lab 3: 2D Correlation experiments (sc) NOTE: in Ullmann B8 (NMR Facility)
(Lab_Notes)
(Lab_3_Problem_Set)

02/23/07 Resonance assignments (mm)
(Lecture_Notes_and_Problem_Set_8)

02/26/07 Resonance assignments & Intro to NMRView

02/28/07 Lab 4: 23 residue antibiotic peptide: 2D correlation spectra & assignments (sc) NOTE: in Ullmann B8 (NMR Facility)
(Lab_Notes)
(Lab_4_Problem_Set)

03/02/07 Structural Constraints & Structure calculation and Refinement (mg)
(Lecture_Notes)

03/05/07 Class cancelled

03/07/07 Lab 5: 2D NOESY spectrum & analysis using NMRVIEW (sc/mg) NOTE: in Belfer Room 1001

03/09/07 Structure evaluation (mg)
(Lecture_Notes)

03/12/07 Nucleic Acids: Assignments, Constraints & Structure (sc)
(Lecture_Notes)

03/14/07 Lab 6: Structure calculation & analysis (mg) NOTE: in Belfer Room 1001
(Lab 6 handout)

03/16/07 Class Cancelled

03/19/07 Representative structure determination papers, via homonuclear methods (mg)

03/21/07 No Lab

03/23/07 Introduction to 3D NMR (via NOESY-HSQC and TOCSY-HSQC) and
Triple resonance experiments and structure determination, Part I (sp)
(Lecture_Notes_and_Problem_Set_9)

03/26/07 Triple resonance experiments and structure determination, Part II (sp)
including labeling schemes, deuteration and example 3D structure determination

03/28/07 Lab 7: 2D & 3D ¹H¹⁵N spectra of labeled proteins (sc/mg) NOTE: in Belfer Room 1001
(Lab_Notes)
(Lab_7_Problem_Set)

03/30/07 Methods for Larger (>30kDa) Proteins (sp)
(Lecture_Notes)

04/02/07 - 04/06/07 No Class for Week

04/09/07 No Class

04/11/07 Lab 8: Triple resonance experiments (sc/mg) NOTE: in Belfer Room 1001
(Lab_Notes)
(Lab_8_Problem_Set)

04/13/07 Heteronuclear relaxation & Macromolecular dynamics (sc)
(Lecture_Notes)

04/16/07 Ionization states & pK_as (mm)
(Lecture_Notes)

04/18/07 Ligand Conformation on Macromolecules (sc)
(Lecture_Notes)

04/18/07 NMR-Based Screening methods in Drug Discovery (sc)
(Lecture_Notes)

04/20/07 "Fun with Enzymes:" k_on, k_off, K_eq, K_eq', PIX, etc. (mg)

04/23/07 Metabolic studies in cells & MRI (lj)
(Lecture_Notes)

04/25/07 Lab 9:Titration of histidine pK_as in ribonuclease (sc) NOTE: in Ullmann B8 (NMR Facility)
(Lab_Notes_and_Lab_9_Problem_Set)

04/27/07 Folding of proteins & nucleic acids (mg)

04/30/07 Water, hydration & hydrogen bonds (sc)

05/02/07 Lab 10:Substrate k_on and k_off from saturation transfer (sc) NOTE: in Ullmann B8 (NMR Facility)