01/9/2012 Introduction, Overview of Course & Visit to the NMR Facility

01/11/2012 Basic Principles of NMR (sc)
Lecture_Notes
Problem_Set_1 - due on 1/13/2012

01/13/2012 Signal Properties and Data Processing (sc)
Lecture_Notes
Problem_Set_2 - due on 1/20/2012

01/16/2012 No Class - Dr Martin Luther King Day

01/18/2012 Chemical Shift and Spin-Spin Coupling and Introduction to T1 and T2 (mg)
Reading
Lecture_Notes
Problem Set 3 - due on 01/23/12

01/20/2012 NMR Lab 1: Introduction & basic 1D experiments (sc) - Location: Ullmann B8
Lab_Notes
Instructions for collecting a 1D 1H NMR spectrum on DRX300
Lab_1_Problem_Set - due on Lab 2, 2/01/2012

01/23/2012 Relaxation & the NOE (mg)
Lecture_Notes
Problem Set 4

01/25/2012 No Class - NYSBDG Meeting at NYAS

01/27/2012 Understanding NMR Experiments Using Product Operators (sc)
Lecture_Notes
Diagram of Product Operators
Product Operator Problem Set - due on 2/3/2012

01/30/2012 A repertoire of one-dimensional methods (mg)
Lecture_Notes

02/01/2012 NMR Lab 2: 1D Homonuclear and Heteronuclear Experiments (sc) - Location: Ullmann B8
Lab_Notes
Instructions for collecting a 1D 13C NMR spectrum on DRX300
Lab_2_Problem_Set - due on Lab 3, 2/10/2012

02/03/2012 Introduction to two-dimensional NMR (mg)
Lecture Notes and Problem Set

02/06/2012 2D Heteronuclear experiments (mg)
Lecture Notes

02/08/2012 2D Homonuclear experiments (mg)
Lecture Notes

02/10/2012 NMR Lab 3: 2D Correlation experiments (sc) - Location: Ullmann B8
Lab_Notes
Lab_3_Problem_Set - due on Lab 4, 2/22/2012

02/13/2012 Introduction to CcpNmr Analysis (computer workshop using Menthol data) (rh)
Lecture Notes
Analysis Session with Menthol Data (12.8Mb)

02/15/2012 Peptide resonance assignments via homonuclear methods (lecture only) (rh)
Reading
Lecture Notes Updated 12 Feb 2012
Problem Set - due on Lab 4, 2/22/2012

02/17/2012 Peptide resonance assignments using CcpNmr Analysis (computer workshop) (rh)
Lecture Notes
Analysis Session with Conotoxin Data (48Mb)

02/20/2012 No Class - President's Day

02/22/2012 NMR Lab 4: 23 residue antibiotic peptide: 2D correlation spectra & assignments (sc) - Location: Ullmann B8
Lab_Notes
Lab_4_Problem_Set - due on Lab 5, 2/29/2012
Analysis Session with Magainin Data (39Mb)

02/24/2012 Pulsed field gradients, Solvent suppression and Self diffusion (mg)
Lecture Notes and Problem Set

02/27/2012 Structural constraints, structure calculation, and evaluation (mg)
Lecture Notes

02/29/2012 NMR Lab 5: 2D NOESY spectrum & analysis using CcpNmr Analysis (lab lecture & computer workshop) (rh/sc)
Lab_Notes
Lab_5_Problem_Set - due on Lab 6, 3/2/2012

03/02/2012 NMR Lab 6: Structure calculation & analysis (computer workshop) (mg)
Lab_Notes and Problem Set - due on 3/9/2012
Analysis Session with Assigned Magainin Data (39Mb)

03/05/2012 Introduction to 3D NMR (via NOESY-HSQC and TOCSY-HSQC) (lecture and computer workshop) (rh)
Lecture Notes
Reading Material
Analysis Session with a 15N labelled peptide (38Mb)

03/07/2012 NMR Lab 7: 2D & 3D ¹H¹⁵N spectra of labeled proteins (lab lecture & computer workshop) (rh/sc)
Using Ubiquitin Analysis Session (1.5Gb!!) during the workshop
Lab_Notes
Lab_7_Problem_Set - due on Lab 8, 3/19/2012
Lab7 Analysis session (9Mb)

03/09/2012 Triple resonance experiments and structure determination, Part I (rh)
Lecture Notes
Problem Set Due on 3/16/12

03/12/2012 Triple resonance experiments and structure determination, Part II (computer workshop) (rh)
Using ubiquitin analysis session - see NMR Lab 7 for download

03/14/2012 Triple resonance experiments and structure determination, Part III (rh)
Lecture Notes - Updated 12 March

03/16/2012 Methods for Larger (>30kDa) Proteins (mg)
Lecture Notes

03/19/2012 NMR Lab 8: Triple resonance experiments (lab lecture & computer workshop) (sc)
Using ubiquitin analysis session in workshop - see NMR Lab 7 for download
Lab_Notes
Lab_8_Problem_Set
Lab8 Analysis session (43Mb)
In Lab Problem Set - Uses Ubiquitin Ananlysis session (see Lab#7 for download)

03/21/2012 Nucleic Acids (sc)
Lecture_Notes

03/23/2012 Representative structure determination papers (mg)

03/26/2012 No Class - Julius Marmur Symposium

03/28/2012 Chemical Exchange in NMR Spectroscopy (sc)
Lecture_Notes

03/30/2012 Heteronuclear relaxation & Macromolecular dynamics, Part I (sc)
Lecture_Notes

04/02/2012 Heteronuclear relaxation & Macromolecular dynamics, Part II (dc)
Reading Material
Reading Material
Reading Material

04/04/2012 Macromolecular Complexes: Structures and Interactions by NMR (eg)
Lecture_Notes

04/06/2012 - 04/13/2012 No Classes Spring Break

04/16/2012 NMR in Ligand Binding Screens and Drug Discovery (eg)
Lecture_Notes

04/18/2012 NMR Lab 9: Mapping Ligand Binding Sites (lab lecture & computer workshop in Belfer 1001) (rh/sc)
Lab_Notes
In Lab_Demo
Lab_9_Problem_Set
Lab9 Analysis session (11Mb)

04/20/2012 Solid State NMR (yt)
Lecture Notes

04/23/2012 Folding of proteins & nucleic acids (mg)
Lecture Notes

04/25/2012 Intrinsically disordered proteins (dc)
Reading Material

04/27/2012 Ionization states & pK_as (mg)
Lecture Notes

04/30/2012 "Fun with Enzymes:" k_on, k_off, K_eq, K_eq', PIX, KIEs, etc. (mg)

05/02/2012 NMR Lab 10: Substrate k_on and k_off from saturation transfer (sc)
Lab_Notes and Problem Set

05/04/2012 In vivo NMR and MRI (lj)
Lecture Notes
Reading Material
Reading Material
Reading Material

05/14/2012 Full Research Proposal Due