The Callender Lab

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    SELECTED RECENT PUBLICATIONS

  • The approach to the Michaelis complex in lactate dehydrogenase: the substrate binding pathway, S. McClendon, N. Zhadin and R. Callender, Biophysical J. 89, 2024-2032 (2005)
  • Structural transformations in the dynamics of Michaelis complex formation in lactate dehydrogenase, S. McClendon, D. Vu, R. Callender and R. B. Dyer, Biophysical J. 89, L07-L09 (2005)
  • Active site contacts in the purine nucleoside phosphorylase/hypoxanthine complex by NMR and ab initio calculations, H. Deng, S. Cahill, A. Lewandowicz, R. Callender, V. Schramm and R. Jones, Biochemistry 43, 15966-15974 (2004)
  • Activating the phosphate nucleophile at the catalytic site of purine nucleoside phosphorylase: a vibrational spectroscopic study, H. Deng, A. Lewandowicz, V. L. Schramm and R. Callender, J Am Chem Soc 126, 9516-7 (2004)
  • Assignment of downfield proton resonances in purine nucleoside phosphorylase immucillin-H complex by saturation-transferred NOEs, H. Deng, A. Lewandowicz, S. M. Cahill, R. H. Furneaux, P. C. Tyler, M. E. Girvin, R. H. Callender and V. L. Schramm, Biochemistry 43, 1980-7 (2004)
  • The assignment of downfield proton resonances in an enzyme inhibitor complex using time-dependent saturation transferred NOEs, H. Deng, S. Cahill, L. Kurz and R. Callender, J Am Chem Soc 126, 1952-3 (2004)
  • Primary folding dynamics of sperm whale apomyoglobin: core formation, M. Gulotta, E. Rogatsky, R. H. Callender and R. B. Dyer, Biophys J 84, 1909-18 (2003)
  • Active site loop motion in triosephosphate isomerase: T-jump relaxation spectroscopy of thermal activation, R. Desamero, S. Rozovsky, N. Zhadin, A. McDermott and R. Callender, Biochemistry 42, 2941-51 (2003)
  • Determination of the ionization state and catalytic function of Glu-133 in peptide deformylase by difference FTIR spectroscopy, H. Deng, R. Callender, J. Zhu, K. T. Nguyen and D. Pei, Biochemistry 41, 10563-9 (2002)
  • Toward an understanding of the role of dynamics on enzymatic catalysis in lactate dehydrogenase, M. Gulotta, H. Deng, R. B. Dyer and R. H. Callender, Biochemistry 41, 3353-63 (2002)
  • Is the PTPase-vanadate complex a true transition state analogue? H. Deng, R. Callender, Z. Huang and Z. Y. Zhang, Biochemistry 41, 5865-72 (2002)
  • Environmental effects on phosphoryl group bonding probed by vibrational spectroscopy: implications for understanding phosphoryl transfer and enzymatic catalysis, H. Cheng, I. Nikolic-Hughes, J. H. Wang, H. Deng, P. J. O'Brien, L. Wu, Z. Y. Zhang, D. Herschlag and R. Callender, J Am Chem Soc 124, 11295-306 (2002)
  • Dynamics of protein ligand binding on multiple time scales: NADH binding to lactate dehydrogenase, H. Deng, N. Zhadin and R. Callender, Biochemistry 40, 3767-73 (2001)
  • Vibrational structure of GDP and GTP bound to RAS: an isotope-edited FTIR study, H. Cheng, S. Sukal, H. Deng, T. S. Leyh and R. Callender, Biochemistry 40, 4035-43 (2001)
  • Vibrational structure of dihydrofolate bound to R67 dihydrofolate reductase, H. Deng, R. Callender and E. Howell, J Biol Chem 276, 48956-60 (2001)
  • gamma-phosphate protonation and pH-dependent unfolding of the Ras.GTP.Mg2+ complex: a vibrational spectroscopy study, H. Cheng, S. Sukal, R. Callender and T. S. Leyh, J Biol Chem 276, 9931-5 (2001)
  • Core formation in apomyoglobin: probing the upper reaches of the folding energy landscape, M. Gulotta, R. Gilmanshin, T. C. Buscher, R. H. Callender and R. B. Dyer, Biochemistry 40, 5137-43 (2001)
  • Structures of apomyoglobin's various acid-destabilized forms, R. Gilmanshin, M. Gulotta, R. B. Dyer and R. H. Callender, Biochemistry 40, 5127-36 (2001)
  • A vibrational structure of 7,8-dihydrobiopterin bound to dihydroneopterin aldolase, H. Deng, R. Callender and G. E. Dale, J Biol Chem 275, 30139-43 (2000)

    • SELECTED REVIEWS

  • Probing protein dynamics using temperature jump relaxation spectroscopy, R. Callender and R. B. Dyer, Curr Opin Struct Biol 12, 628-33 (2002)
  • Raman spectroscopic studies of the structures, energetics, and bond distortions of substrates bound to enzymes, H. Deng and R. Callender, Methods Enzymol 308, 176-201 (1999)
  • Fast events in protein folding: the time evolution of primary processes, R. H. Callender, R. B. Dyer, R. Gilmanshin and W. H. Woodruff, Annu Rev Phys Chem 49, 173-202 (1998)
  • Infrared studies of fast events in protein folding, R. Brian Dyer, Feng Gai, William H. Woodruff, Rudolf Gilmanshin, and Robert Callender, Accs. Chem. Res. 31, 709-716 (1998)
  • Nonresonance Raman difference spectroscopy: a general probe of protein structure, ligand binding, enzymatic catalysis, and the structures of other biomacromolecules, R. Callender and H. Deng, Annu Rev Biophys Biomol Struct 23, 215-45 (1994)

    • SELECTED PREVIOUS PUBLICATIONS (over 130 total peer reviewed articles)

  • Characterization of hydrogen bonding in the complex of adenosine deaminase with a transition state analogue: a Raman spectroscopic study, H. Deng, L. C. Kurz, F. B. Rudolph and R. Callender, Biochemistry 37, 4968-76 (1998)
  • Raman difference spectroscopic studies of the myosin S1.MgADP.vanadate complex, H. Deng, J. Wang, R. H. Callender, J. C. Grammer and R. G. Yount, Biochemistry 37, 10972-9 (1998)
  • Raman difference studies of GDP and GTP binding to c-Harvey ras, J. H. Wang, D. G. Xiao, H. Deng, M. R. Webb and R. Callender, Biochemistry 37, 11106-16 (1998)
  • The core of apomyoglobin E-form folds at the diffusion limit, R. Gilmanshin, R. H. Callender and R. B. Dyer, Nat Struct Biol 5, 363-5 (1998)
  • A Raman spectroscopic characterization of bonding in the complex of horse liver alcohol dehydrogenase with NADH and N-cyclohexylformamide, H. Deng, J. F. Schindler, K. B. Berst, B. V. Plapp and R. Callender, Biochemistry 37, 14267-78 (1998)
  • Fast events in protein folding: relaxation dynamics of secondary and tertiary structure in native apomyoglobin, R. Gilmanshin, S. Williams, R. H. Callender, W. H. Woodruff and R. B. Dyer, Proc Natl Acad Sci U S A 94, 3709-13 (1997)
  • Fast events in protein folding: relaxation dynamics and structure of the I form of apomyoglobin, R. Gilmanshin, S. Williams, R. H. Callender, W. H. Woodruff and R. B. Dyer, Biochemistry 36, 15006-12 (1997)
  • The contribution of electrostatic and van der Waals interactions to the stereospecificity of the reaction catalyzed by lactate dehydrogenase, J. van Beek, R. Callender and M. R. Gunner, Biophys J 72, 619-26 (1997)
  • pH-dependent conformational changes in Escherichia coli dihydrofolate reductase revealed by Raman difference spectroscopy, Y. Q. Chen, J. Kraut and R. Callender, Biophys J 72, 936-41 (1997)
  • Structural heterogeneity of the various forms of apomyoglobin: implications for protein folding, R. Gilmanshin, R. B. Dyer and R. H. Callender, Protein Sci 6, 2134-42 (1997)
  • Fast events in protein folding: helix melting and formation in a small peptide, S. Williams, T. P. Causgrove, R. Gilmanshin, K. S. Fang, R. H. Callender, W. H. Woodruff and R. B. Dyer, Biochemistry 35, 691-7 (1996)
  • Trypanosomal nucleoside hydrolase. Resonance Raman spectroscopy of a transition-state inhibitor complex, H. Deng, A. W. Chan, C. K. Bagdassarian, B. Estupinan, B. Ganem, R. H. Callender and V. L. Schramm, Biochemistry 35, 6037-47 (1996)
  • Evidence for a bound water molecule next to the retinal Schiff base in bacteriorhodopsin and rhodopsin: a resonance Raman study of the Schiff base hydrogen/deuterium exchange, H. Deng, L. Huang, R. Callender and T. Ebrey, Biophys J 66, 1129-36 (1994)
  • Hydrogen bond interactions of G proteins with the guanine ring moiety of guanine nucleotides, G. Weng, C. X. Chen, V. Balogh-Nair, R. Callender and D. Manor, Protein Sci 3, 22-9 (1994)
  • Determination by Raman spectroscopy of the pKa of N5 of dihydrofolate bound to dihydrofolate reductase: mechanistic implications, Y. Q. Chen, J. Kraut, R. L. Blakley and R. Callender, Biochemistry 33, 7021-6 (1994)
  • Source of catalysis in the lactate dehydrogenase system. Ground-state interactions in the enzyme-substrate complex, H. Deng, J. Zheng, A. Clarke, J. J. Holbrook, R. Callender and J. W. Burgner, 2nd, Biochemistry 33, 2297-305 (1994)
  • Structure of the complex between pyridoxal 5'-phosphate and the tyrosine 225 to phenylalanine mutant of Escherichia coli aspartate aminotransferase determined by isotope-edited classical Raman difference spectroscopy, J. M. Goldberg, J. Zheng, H. Deng, Y. Q. Chen, R. Callender and J. F. Kirsch, Biochemistry 32, 8092-7 (1993)
  • Comparison of vibrational frequencies of critical bonds in ground-state complexes and in a vanadate-based transition-state analog complex of muscle phosphoglucomutase. Mechanistic implications, H. Deng, W. J. Ray, Jr., J. W. Burgner, 2nd and R. Callender, Biochemistry 32, 12984-92 (1993)
  • A vibrational analysis of the catalytically important C4-H bonds of NADH bound to lactate or malate dehydrogenase: ground-state effects, H. Deng, J. Zheng, D. Sloan, J. Burgner and R. Callender, Biochemistry 31, 5085-92 (1992)
  • Ultrafast spectroscopy of the visual pigment rhodopsin, M. Yan, D. Manor, G. Weng, H. Chao, L. Rothberg, T. M. Jedju, R. R. Alfano and R. H. Callender, Proc Natl Acad Sci U S A 88, 9809-12 (1991)
  • An isotope edited classical Raman difference spectroscopic study of the interactions of guanine nucleotides with elongation factor Tu and H-ras p21, D. Manor, G. Z. Weng, H. Deng, S. Cosloy, C. X. Chen, V. Balogh-Nair, K. Delaria, F. Jurnak and R. Callender, Biochemistry 30, 10914-20 (1991)
  • Molecular properties of pyruvate bound to lactate dehydrogenase: a Raman spectroscopic study, H. Deng, J. Zheng, J. Burgner and R. Callender, Proc Natl Acad Sci U S A 86, 4484-8 (1989)
  • Molecular properties of p-(dimethylamino)benzaldehyde bound to liver alcohol dehydrogenase: a Raman spectroscopic study, R. Callender, D. Chen, J. Lugtenburg, C. Martin, K. W. Rhee, D. Sloan, R. Vandersteen and K. T. Yue, Biochemistry 27, 3672-81 (1988)
  • Resonance Raman spectroscopy of octopus rhodopsin and its photoproducts, C. Pande, A. Pande, K. T. Yue, R. Callender, T. G. Ebrey and M. Tsuda, Biochemistry 26, 4941-7 (1987)
  • Resonance raman spectroscopy of an ultraviolet-sensitive insect rhodopsin, C. Pande, H. Deng, P. Rath, R. H. Callender and J. Schwemer, Biochemistry 26, 7426-30 (1987)
  • A study of the Schiff base mode in bovine rhodopsin and bathorhodopsin, H. Deng and R. H. Callender, Biochemistry 26, 7418-26 (1987)
  • The primary event in vision investigated by time-resolved fluorescence spectroscopy, A. G. Doukas, M. R. Junnarkar, R. R. Alfano, R. H. Callender and V. Balogh-Nair, Biophys J 47, 795-8 (1985)
  • Fluorescence quantum yield of visual pigments: evidence for subpicosecond isomerization rates, A. G. Doukas, M. R. Junnarkar, R. R. Alfano, R. H. Callender, T. Kakitani and B. Honig, Proc Natl Acad Sci U S A 81, 4790-4 (1984)
  • Acid-base equilibrium of the Schiff base in bacteriorhodopsin, S. Druckmann, M. Ottolenghi, A. Pande, J. Pande and R. H. Callender, Biochemistry 21, 4953-9 (1982)
  • On the mechanism of hydrogen-deuterium exchange in bacteriorhodopsin, A. G. Doukas, A. Pande, T. Suzuki, R. H. Callender, B. Honig and M. Ottolenghi, Biophys J 33, 275-9 (1981)
  • Photochemistry of rhodopsin and isorhodopsin investigated on a picosecond time scale, T. G. Monger, R. R. Alfano and R. H. Callender, Biophys J 27, 105-15 (1979)
  • Photoisomerization, energy storage, and charge separation: a model for light energy transduction in visual pigments and bacteriorhodopsin, B. Honig, T. Ebrey, R. H. Callender, U. Dinur and M. Ottolenghi, Proc Natl Acad Sci U S A 76, 2503-7 (1979)
  • Photochemical cis-trans isomerisation of bovine rhodopsin at liquid helium temperatures, B. Aton, R. H. Callender and B. Honig, Nature 273, 784-6 (1978)
  • Resonance Raman studies of visual pigments, R. Callender, Annu Rev Biophys Bioeng 6, 33-55 (1977)
  • Cis-trans isomerisation in rhodopsin occurs in picoseconds, B. H. Green, T. G. Monger, R. R. Alfano, B. Aton and R. H. Callender, Nature 269, 179-80 (1977)
  • Molecular flow resonance Raman effect from retinal and rhodopsin, R. H. Callender, A. Doukas, R. Crouch and K. Nakanishi, Biochemistry 15, 1621-9 (1976)
  • Resonance Raman spectroscopy of rhodopsin in retinal disk membranes, A. R. Oseroff and R. H. Callender, Biochemistry 13, 4243-8 (1974)
  • Dependence of the transition moment on internuclear separation, R. H. Callender, J. I. Gersten, R. W. Leigh and J. L. Yang, Phys. Rev. Letters 32, 917 (1974)
  • Role of metastable dimers in the theory of induced atomic fluorescence, R. H. Callender, J. I. Gersten, R. W. Leigh and J. L. Yang, Phys. Rev. Letters 33, 1311 (1974)
  • Rotational Raman Effect: molecular impurities in alkali halides, R. H. Callender and P. S. Pershan, Phys. Rev. A2, 672 (1970)
  • Molecular rotations and kinetics by light scattering, R. H. Callender and P. S. Pershan, Phys. Rev. Letters 23, 947 (1969)
  • The response of high altitude ionization chambers during 1954-1966 solar cycle, R. H. Callender, J. R. Manzano and J. Winckler, J. Geophysical Res. 70, 3189 (1965)