Blanchard Lab
Department of Biochemistry
315 Forchheimer Bldg.
1300 Morris Park Avenue
Bronx, NY 10461-1602
John Blanchard

(718) 430-3096
blanchar@aecom.yu.edu

References

Tremblay LW, Blanchard JS. (2009) "The 1.9 Å structure of the branched-chain amino-acid transaminase (IlvE) from Mycobacterium tuberculosis." Acta Crystallogr Sect F Struct Biol Cryst Commun. 65(Pt 11):1071-1077.

Stoll VS, Blanchard JS. (2009) "Buffers: principles and practice." Methods Enzymol. 2009;463:43-56.

Sikora AL, Cahill SM, Blanchard JS. (2009) "Enterobactin synthetase-catalyzed formation of P(1),P(3)-diadenosine-5'-tetraphosphate." Biochemistry. 48(46):10827-9

Fan F, Vetting MW, Frantom PA, Blanchard JS. (2009) "Structures and mechanisms of the mycothiol biosynthetic enzymes." Curr Opin Chem Biol. 13(4):444-52.

Frantom PA, Zhang HM, Emmett MR, Marshall AG, Blanchard JS. (2009) "Mapping of the allosteric network in the regulation of alpha-isopropylmalate synthase from Mycobacterium tuberculosis by the feedback inhibitor L-leucine: solution-phase H/D exchange monitored by FT-ICR mass spectrometry." Biochemistry. 48(31):7457-64.

Fan F, Blanchard JS. (2009) "Toward the catalytic mechanism of a cysteine ligase (MshC) from Mycobacterium smegmatis: an enzyme involved in the biosynthetic pathway of mycothiol." Biochemistry. 48(30):7150-9.

Reich-Slotky R, Kabbash CA, Della-Latta P, Blanchard JS, Feinmark SJ, Freeman S, Kaplan G, Shuman HA, Silverstein SC. (2009) " Gemfibrozil inhibits Legionella pneumophila and Mycobacterium tuberculosis enoyl coenzyme A reductases and blocks intracellular growth of these bacteria in macrophages."
J Bacteriol. 191(16):5262-71.

Vetting MW, Hegde SS, Blanchard JS. (2009) "Crystallization of a pentapeptide-repeat protein by reductive cyclic pentylation of free amines with glutaraldehyde. Acta Crystallogr D Biol Crystallogr. 65(Pt 5), 462-9.

Hugonnet JE, Tremblay LW, Boshoff HI, Barry CE 3rd, Blanchard JS. (2009) "Meropenem-clavulanate is effective against extensively drug-resistant Mycobacterium tuberculosis." Science. 323(5918), 1215-8.

de Carvalho LP, Frantom PA, Argyrou A, Blanchard JS. (2009) " Kinetic Evidence for Interdomain Communication in the Allosteric Regulation of alpha-Isopropylmalate Synthase from Mycobacterium tuberculosis." Biochemistry, 2009, 48 (9), pp 1996–2004

Tremblay LW, Fan F, Vetting MW, Blanchard JS (2008) "The 1.6 A crystal structure of Mycobacterium smegmatis MshC: the penultimate enzyme in the mycothiol biosynthetic pathway."
Biochemistry. 47(50):13326-35.

Khrapunov S, Cheng H, Hegde S, Blanchard J, Brenowitz M. (2008) "Solution structure and refolding of the Mycobacterium tuberculosis pentapeptide repeat protein MfpA." J Biol Chem. 283(52):36290-9

Vetting MW, Errey JC, Blanchard JS. (2008) " Rv0802c from Mycobacterium tuberculosis: the first structure of a succinyltransferase with the GNAT fold."
Acta Crystallogr Sect F Struct Biol Cryst Commun. 64(Pt 11):978-85

Sikora AL, Frankel BA, Blanchard JS. (2008) " Kinetic and chemical mechanism of arylamine N-acetyltransferase from Mycobacterium tuberculosis." Biochemistry. 47(40):10781-9.

Vetting MW, Park CH, Hegde SS, Jacoby GA, Hooper DC, Blanchard JS. (2008) "Mechanistic and structural analysis of aminoglycoside N-acetyltransferase AAC(6')-Ib and its bifunctional, fluoroquinolone-active AAC(6')-Ib-cr variant." Biochemistry. 47(37):9825-35.

Vetting MW, Bareich DC, Yu M, Blanchard JS. (2008) "Crystal structure of RimI from Salmonella typhimurium LT2, the GNAT responsible for N(alpha)-acetylation of ribosomal protein S18." Protein Sci. 17(10):1781-90.

Frankel BA, Blanchard JS. (2008) "Mechanistic analysis of Mycobacterium tuberculosis Rv1347c, a lysine Nepsilon-acyltransferase involved in mycobactin biosynthesis." Arch Biochem Biophys. 477(2):259-66.

Magalhães ML, Argyrou A, Cahill SM, Blanchard JS. (2008) "Kinetic and mechanistic analysis of the Escherichia coli ribD-encoded bifunctional deaminase-reductase involved in riboflavin biosynthesis."
Biochemistry. 47(24):6499-507

Tremblay LW, Hugonnet JE, Blanchard JS. (2008) "Structure of the Covalent Adduct Formed between Mycobacterium tuberculosis beta-Lactamase and Clavulanate." Biochemistry. 47(19), 5312-6.

Vetting MW, Frantom PA, Blanchard JS. (2008) "Structural and enzymatic analysis of MshA from corynebacterium glutamicum: Substrate-assisted catalysis." J Biol Chem. 283(23):15834-44.

Williams L, Fan F, Blanchard JS, Raushel FM. (2008) Positional Isotope Exchange Analysis of the Mycobacterium smegmatis Cysteine Ligase (MshC). Biochemistry. 47(16):4843-50.

Magalhães ML, Vetting MW, Gao F, Freiburger L, Auclair K, Blanchard JS. (2008) "Kinetic and Structural Analysis of Bisubstrate Inhibition of the Salmonella enterica Aminoglycoside 6'-N-Acetyltransferase." Biochemistry. 47(2):579-84.

Hugonnet JE, Blanchard JS. (2007) "Irreversible inhibition of the Mycobacterium tuberculosis beta-lactamase by clavulanate." Biochemistry. 46(43):11998-2004

Pillai B, Cherney M, Diaper CM, Sutherland A, Blanchard JS, Vederas JC, James MN. (2007) "Dynamics of catalysis revealed from the crystal structures of mutants of diaminopimelate epimerase."
Biochem Biophys Res Commun. 363(3):547-53.

Fan F, Luxenburger A, Painter GF, Blanchard JS. (2007) "Steady-state and pre-steady-state kinetic analysis of Mycobacterium smegmatis cysteine ligase (MshC)." Biochemistry. 46(40):11421-9.

Argyrou A, Vetting MW, Blanchard JS. (2007) "New insight into the mechanism of action of and resistance to isoniazid: interaction of Mycobacterium tuberculosis enoyl-ACP reductase with INH-NADP."
J Am Chem Soc. 129(31):9582-3.

Hegde SS, Chandler J, Vetting MW, Yu M, Blanchard JS. (2007) "Mechanistic and structural analysis of human spermidine/spermine N1-acetyltransferase." Biochemistry. 46(24):7187-95. .

Vetting MW, Hegde SS, Hazleton KZ, Blanchard JS.
(2007) "Structural characterization of the fusion of two pentapeptide repeat proteins, Np275 and Np276, from Nostoc punctiforme: resurrection of an ancestral protein." Protein Sci. 16(4):755-60.

Yu M, Magalhaes ML, Cook PF, Blanchard JS. (2006) " Bisubstrate inhibition: Theory and application to N-acetyltransferases." Biochemistry. 45(49):14788-94.


Yu M, de Carvalho LP, Sun G, Blanchard JS. (2006) "Activity-based substrate profiling for Gcn5-related N-acetyltransferases: the use of chloroacetyl-coenzyme A to identify protein substrates."
J Am Chem Soc. 128(48):15356-7.

Argyrou A, Jin L, Siconilfi-Baez L, Angeletti RH, Blanchard JS. (2006) "Proteome-wide profiling of isoniazid targets in Mycobacterium tuberculosis." Biochemistry. 45(47):13947-53.


Silva RG, de Carvalho LP, Blanchard JS, Santos DS, Basso LA. (2006) "Mycobacterium tuberculosis beta-Ketoacyl-Acyl Carrier Protein (ACP) Reductase: Kinetic and Chemical Mechanisms."
Biochemistry. 31;45(43):13064-13073.

Wing C, Errey JC, Mukhopadhyay B, Blanchard JS, Field RA. (2006) "Expression and initial characterization of WbbI, a putative d-Galf:alpha-d-Glc beta-1,6-galactofuranosyltransferase from Escherichia coli K-12." Org Biomol Chem. 4(21):3945-50.

de Carvalho LP, Blanchard JS. (2006) "Kinetic and chemical mechanism of alpha-isopropylmalate synthase from Mycobacterium tuberculosis." Biochemistry. 45(29):8988-99.

Pillai B, Cherney MM, Diaper CM, Sutherland A, Blanchard JS, Vederas JC, James MN. (2006) "Structural insights into stereochemical inversion by diaminopimelate epimerase: an antibacterial drug target." Proc Natl Acad Sci U S A. 103(23):8668-73.

de Carvalho LP, Blanchard JS. (2006) "Kinetic analysis of the effects of monovalent cations and divalent metals on the activity of Mycobacterium tuberculosis alpha-isopropylmalate synthase."
Arch Biochem Biophys. 451(2):141-8.

Argyrou A, Vetting MW, Aladegbami B, Blanchard JS. (2006) "Mycobacterium tuberculosis dihydrofolate reductase is a target for isoniazid." Nat Struct Mol Biol. 13: 408 - 413

Errey JC, Blanchard JS. (2006) "Functional Annotation and Kinetic Characterization of PhnO from Salmonella enterica." Biochemistry. 45(9):3033-9.

Vetting MW, Hegde SS, Fajardo JE, Fiser A, Roderick SL, Takiff HE, Blanchard JS. (2006) "Pentapeptide Repeat Proteins." Biochemistry. 45(1):1-10

Vetting MW, Yu M, Rendle PM, Blanchard JS. (2006) "The Substrate-induced Conformational Change of Mycobacterium tuberculosis Mycothiol Synthase." J Biol Chem. 281(5):2795-802

Magalhaes ML, Blanchard JS. (2005) "The kinetic mechanism of AAC3-IV aminoglycoside acetyltransferase from Escherichia coli." Biochemistry. 44(49):16275-83

Diaper CM, Sutherland A, Pillai B, James MN, Semchuk P, Blanchard JS, Vederas JC. (2005) "The stereoselective synthesis of aziridine analogues of diaminopimelic acid (DAP) and their interaction with dap epimerase." Org Biomol Chem. 3(24):4402-11.

Blanchard JS. (2005) "Old approach yields new antibiotic." Nat Med. 11(10):1045-6.

de Carvalho LP, Argyrou A, Blanchard JS. (2005) "Slow-onset feedback inhibition: inhibition of Mycobacterium tuberculosis alpha-isopropylmalate synthase by L-leucine."
J Am Chem Soc. 127(28):10004-5.

Hegde SS, Vetting MW, Roderick SL, Mitchenall LA, Maxwell A, Takiff HE, Blanchard JS. (2005)
"A fluoroquinolone resistance protein from Mycobacterium tuberculosis that mimics DNA."
Science. 308(5727):1480-3.

Errey JC, Blanchard JS. (2005) "Functional characterization of a novel ArgA from Mycobacterium tuberculosis." J Bacteriol. 187(9):3039-44.

Vetting MW, de Carvalho LP, Roderick SL, Blanchard JS. (2005) "A Novel Dimeric Structure of the RimL Na-acetyltransferase from Salmonella typhimurium." J Biol Chem. 280(23):22108-14.

Vetting MW, S de Carvalho LP, Yu M, Hegde SS, Magnet S, Roderick SL, Blanchard JS. (2005) "Structure and functions of the GNAT superfamily of acetyltransferases.
Arch Biochem Biophys. 433(1):212-26.

Guillemot D, Bonacorsi S, Blanchard JS, Weber P, Simon S, Guesnon B, Bingen E, Carbon C. (2004) "Amoxicillin-clavulanate therapy increases childhood nasal colonization by methicillin-susceptible Staphylococcus aureus strains producing high levels of penicillinase.
Antimicrob Agents Chemother. 48(12):4618-23.

Argyrou A, Vetting MW, Blanchard JS. (2004) "Characterization of a new member of the flavoprotein disulfide reductase family of enzymes from mycobacterium tuberculosis."
J Biol Chem. 279(50):52694-702.

Argyrou A, Blanchard JS. (2004) "Flavoprotein disulfide reductases: advances in chemistry and function."
Prog Nucleic Acid Res Mol Biol. 78:89-142.

Zheng R, Dam TK, Brewer CF, Blanchard JS. (2004) "Residues in Mycobacterium tuberculosis Pantothenate Synthetase Required in the Formation and Stabilization of the Adenylate Intermediate."
Biochemistry. 8;43(22):7171-8.

Vetting MW, Magnet S, Nieves E, Roderick SL, Blanchard JS. (2004) "A bacterial acetyltransferase capable of regioselective N-acetylation of antibiotics and histones." Chem Biol. Apr;11(4):565-73.

Argyrou A, Blanchard JS. (2004) "Kinetic and chemical mechanism of Mycobacterium tuberculosis 1-deoxy-D-xylulose-5-phosphate isomeroreductase." Biochemistry, 43(14):4375-84.

Disney MD, Magnet S, Blanchard JS, Seeberger PH. (2004) "Aminoglycoside microarrays to study antibiotic resistance." Angew Chem Int Ed Engl. 43(12):1591-4.

Magnet S, Blanchard JS. (2004) "Mechanistic and kinetic study of the ATP-dependent DNA ligase of Neisseria meningitidis." Biochemistry, 43(3):710-7.

Zheng R, Blanchard JS. (2003) "Substrate specificity and kinetic isotope effect analysis of the Eschericia coli ketopantoate reductase." Biochemistry, 42(38):11289-96

Cirilli M, Zheng R, Scapin G, Blanchard JS. (2003). "The three-dimensional structures of the Mycobacterium tuberculosis dihydrodipicolinate reductase-NADH-2,6-PDC and -NADPH-2,6-PDC complexes. Structural and mutagenic analysis of relaxed nucleotide specificity."
Biochemistry. 42(36):10644-50.

Vetting MW, Roderick SL, Yu M, Blanchard JS. (2003). "Crystal structure of mycothiol synthase (Rv0819) from Mycobacterium tuberculosis shows structural homology to the GNAT family of N-acetyltransferases."
Protein Science, 12(9):1954-9

Vogt RN, Steenkamp DJ, Zheng R, Blanchard JS. (2003). "The metabolism of nitrosothiols in the Mycobacteria: identification and characterization of S-nitrosomycothiol reductase."
Biochem J., 374(Pt 3):657-66.

Vetting MW, Roderick SL, Hegde S, Magnet S, Blanchard JS. (2003). "What can structure tell us about in vivo function? The case of aminoglycoside-resistance genes."
Biochem Soc. Trans.31(Pt 3):520-2.

Williams L, Zheng R, Blanchard JS, Raushel FM. (2003). "Positional isotope exchange analysis of the pantothenate synthetase reaction." Biochemistry, 42(17):5108-13.

Magnet S, Smith TA, Zheng R, Nordmann P, Blanchard JS. (2003) "Aminoglycoside resistance resulting from tight drug binding to an altered aminoglycoside acetyltransferase." Antimicrob Agents Chemother. 47(5):1577-83.

Hegde SS, Blanchard JS. (2003). "Kinetic and mechanistic characterization of recombinant Lactobacillus viridescens FemX (UDP-N-acetylmuramoyl pentapeptide-lysine N6-alanyltransferase)."
J Biol Chem., 278(25):22861-7.

Blanchard JS. (2003) "Resisting bacterial drug resistance." Chem Biol, 10(2):104-6.

Argyrou A, Sun G, Palfey BA, Blanchard JS. (2003) "Catalysis of diaphorase reactions by Mycobacterium tuberculosis lipoamide dehydrogenase occurs at the EH4 level." Biochemistry, 42(7):2218-28.

Sugantino M, Zheng R, Yu M, Blanchard JS. (2003), "Mycobacterium tuberculosis ketopantoate hydroxymethyltransferase: tetrahydrofolate-independent hydroxymethyltransferase and enolization reactions with alpha-keto acids." Biochemistry, 42(1):191-9.

Argyrou A, Blanchard JS, Palfey BA. (2002) "The lipoamide dehydrogenase from Mycobacterium tuberculosis permits the direct observation of flavin intermediates in catalysis."
Biochemistry, 41(49):14580-90.

Schroeder EK, de Souza N, Santos DS, Blanchard JS, Basso LA. (2002). Drugs that inhibit mycolic acid biosynthesis in Mycobacterium tuberculosis. Curr Pharm Biotechnol, 3(3):197-225.

Vetting MW, Hegde SS, Javid-Majd F, Blanchard JS, Roderick SL. (2002). Aminoglycoside 2'-N-acetyltransferase from Mycobacterium tuberculosis in complex with coenzyme A and aminoglycoside substrates.
Nat Struct Biol, 9(9):653-8.

Hegde SS, Dam TK, Brewer CF, Blanchard JS. (2002) "Thermodynamics of aminoglycoside and acyl-coenzyme A binding to the Salmonella enterica AAC(6')-Iy aminoglycoside N-acetyltransferase." Biochemistry. 41(23):7519-27.

Argyrou A, Blanchard JS. (2001) "Mycobacterium tuberculosis lipoamide dehydrogenase is encoded by Rv0462 and not by the lpdA or lpdB genes."
Biochemistry, 40(38):11353-63.
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Magnet S, Lambert T, Courvalin P, Blanchard JS. (2001) "Kinetic and mutagenic characterization of the chromosomally encoded Salmonella enterica AAC(6')-Iy aminoglycoside N-acetyltransferase."
Biochemistry. 40(12):3700-9.

Hegde SS, Javid-Majd F, Blanchard JS. (2001) "Overexpression and mechanistic analysis of chromosomally encoded aminoglycoside 2'-N-acetyltransferase (AAC(2')-Ic) from Mycobacterium tuberculosis." J Biol Chem. 276(49):45876-81.

Zheng R, Blanchard JS. (2001) "Steady-state and pre-steady-state kinetic analysis of Mycobacterium tuberculosis pantothenate synthetase." Biochemistry, 40(43):12904-12.

Zheng R, Blanchard JS. (2000) "Identification of active site residues in E. coli ketopantoate reductase by mutagenesis and chemical rescue." Biochemistry. 39(51):16244-51.

Caplan JF, Zheng R, Blanchard JS, Vederas JC. (2000) "Vinylogous amide analogues of diaminopimelic acid (DAP) as inhibitors of enzymes involved in bacterial lysine biosynthesis." Org Lett. 2(24):3857-60.

Zheng R, Blanchard JS. (2000) "Kinetic and mechanistic analysis of the E. coli panE-encoded ketopantoate reductase. Biochemistry. 39(13):3708-17.

Cirilli M, Zheng R, Scapin G, Blanchard JS. (1998) "Structural symmetry: the three-dimensional structure of Haemophilus influenzae diaminopimelate epimerase." Biochemistry. 37(47):16452-8.

Basso LA, Zheng R, Musser JM, Jacobs WR Jr, Blanchard JS. (1998) "Mechanisms of isoniazid resistance in Mycobacterium tuberculosis: enzymatic characterization of enoyl reductase mutants identified in isoniazid-resistant clinical isolates. J Infect Dis. 178(3):769-75.

Born TL, Zheng R, Blanchard JS. (1998) "Hydrolysis of N-succinyl-L,L-diaminopimelic acid by the Haemophilus influenzae dapE-encoded desuccinylase: metal activation, solvent isotope effects, and kinetic mechanism." Biochemistry. 21;37(29):10478-87.

Scapin G, Reddy SG, Zheng R, Blanchard JS. (1997) "Three-dimensional structure of Escherichia coli dihydrodipicolinate reductase in complex with NADH and the inhibitor 2,6-pyridinedicarboxylate."
Biochemistry. 36(49):15081-8.

Zheng R, Cenas N, Blanchard JS. (1995) "Catalytic and potentiometric characterization of E201D and E201Q mutants of Trypanosoma congolense trypanothione reductase." Biochemistry. 34(39):12697-703.